Why the cut-off depth of fluorescent filter should be OD6

Why the cut-off depth of fluorescent filter should be OD6

In recent years, with the development of life science technology, fluorescence probe technology

Fluorescent microscope, Polarization fluorescence detection technology, Multiphoton fluorescence detection technology

luorescent filter

PCR gene amplification, fluorescence quantitative detection, etc

Biomedical detection technologies are constantly emerging,

For the gradient of the fluorescent filter,

The background depth puts forward higher technical requirements.

The duration of stimulated emission fluorescence of fluorescent reagent is very short

Emitting fluorescence brightness equivalent to excitation energy

One thousandth to one thousandth of a magnitude

Compared with the excited fluorescence, it is very weak

Both coexist in the test sample

And the excitation spectrum and emission spectrum wavelength gap is very small (about 15nm-30nm)

So we need to detect the light signal to excite the light in the fluorescence system

Deep isolation with emission light in the spectrum

Isolation degree reaches above OD4

To detect the fluorescent signal emitted by the sample

To improve the accuracy of fluorescence detection

Need to improve the isolation to above OD5

And the higher the isolation of the filter

The stronger the detection ability of micro fluorescence

The higher the detection accuracy of fluorescence microscope

So the core requirement of the filter of the fluorescence microscope is

High cut-off gradient, high transmittance of the filter, and cut-off of the background near the filter.

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