Fluorescence Microscope Filters
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INOUIRY
A set of falling light fluorescence microscope filter set consists of excitation filter EX, dichroic mirror Dicroic, and emission filter EM, all of which are indispensable:
1. Excitation (EX) of the filter
Position: Between the light source and the dichroic mirror
Function: Filter narrow bands of light sources, only release light that can excite fluorescent dyes, and block other miscellaneous light
Type: Almost all bandpass BP, labeling format: center wavelength/half width, such as 480/30 (465~495nm transparent)
Key indicators: Peak transmittance>85%, cut-off OD ≥ 4 (attenuation of stray light by more than 10000 times)
2. Dichroic dichroic mirror
Installation angle: Fixed 45 ° optical path
Function: Short wave reflection, long wave transmission; Reflect the excitation light towards the sample, and the sample emits longer wavelength fluorescence that directly penetrates the lens
Requirements: steep spectral edges, mirror surface flatness (to avoid image distortion), low wavefront distortion
3. Emission filter (EM/Carrier)
Position: From the two-way mirror to the camera/eyepiece at the last turn
Function: Thoroughly intercept residual excitation light, sample scattered light, and spontaneous fluorescence, and only release target fluorescence signals
Type: Bandpass BP (single dye precision imaging)/Longpass LP (wideband observation)
Definition of Filter Spectral Types
1. BP bandpass (most commonly used): Only through a certain interval, EX and EM mainstream selection
2. LP Long Pass: Transmitting all light with a wavelength greater than the critical wavelength, multi-color imaging, and easy use of fluorescent mirrors
3. SP short circuit: Passing through<critical wavelength, fluorescent devices are rarely used alone
Mainstream standard filter set (corresponding to commonly used fluorescent dyes)
Product specifications:
Center wavelength: 350-850nm optional
Half wave bandwidth: 10-100nm optional
Closing Background:> 0D6@300-1100nm
Product specifications: D25 × 3.5; D25.4×3.5; D25×6; customizable
| Filter Group | Dye | Excitation EX (nm) | Dichroic Mirror Cutoff (nm) | Emission EM (nm) |
|---|---|---|---|---|
| DAPI/Blue | nucleus DAPI、Hoechst | 360/40 | 400 | 460/50 |
| FITC/Green | FITC、AF488、GFP | 480/30 | 505 | 525/50 |
| TRITC/Red | TRITC、Cy3、AF555 | 550/25 | 570 | 600/50 |
| Texas Red | Texas Red、AF594 | 580/30 | 595 | 630/60 |
| Cy5 infrared | Cy5、AF647 | 630/30 | 660 | 680/50 |
Key technical parameters
1. Transmittance T: The peak transmittance of the working band is ≥ 80%, and the higher the transmittance, the stronger the fluorescence brightness
2. Cut off depth OD
Ordinary laboratory: OD4 (10 ⁻⁴ transparent)
High sensitivity/weak fluorescence, multi-color co dyeing: OD6 (10 ⁻⁶ transparent, no background noise)
3. Steep spectral edge: The narrower the transition interval, the better to reduce dye cross color
There are some stock models available for selection above, if you need custom Optical Fluorescence microscope Filters or bulk orders, please contact our sales team:
info@borisuns.com
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