Enzyme Linked Immunosorbent Assay Filters
There are two main routes for enzyme-linked immunosorbent assay (ELISA/ELISA) filters: colorimetric ELISA (the most popular transmission absorption method) and fluorescent enzyme-linked immunosorbent assay (F-ELISA).
1) Colorimetric ELISA (ordinary enzyme-linked immunosorbent assay, TMB substrate)
Industry standard wavelength: 450 nm
The maximum absorption peak after HRP+TMB substrate termination reaction is included as standard in 80% commercial ELISA kits: 492 nm
Classical wavelength of OPD substrate: 405 nm
AP enzyme/PNPP substrate: 620/630 nm
Universal for mass production: Φ 8/Φ 10mm
2) Fluorescent enzyme-linked immunosorbent assay (fluorescent enzyme-linked immunosorbent assay, multifunctional enzyme-linked immunosorbent assay)
Falling fluorescent light path, paired use: excitation filter Excitation+emission filter Emission, optional dichroic mirror Dicroic
Common fluorescent channels:
FITC:Ex488 / Em525
Cy3/TAMRA:Ex550 / Em578
Cy5:Ex630 / Em665
Recommended combinations of common solutions
Plan A | Basic Standard Version (Universal Desktop ELISA reader)
BP405、BP450、BP492、BP630; FWHM10nm,Φ10×2.0mm
Plan B | Simplified Economy Type (Large Batch POCT Enzyme Exemption)
BP450 (main)+BP630 (reference) dual channel
Scheme C | Multi functional fluorescent enzyme immunoassay (excitation+emission paired filter)
488/525, 550/578 and other combinations, paired with 450nm colorimetric channel
The above models are available in stock for you to choose from, if you need custom Optical ELISA Filters or bulk orders, please contact our sales team:
info@borisuns.com
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